Set 12 2013
Meat Juice Multiserology – Developing a protein microarray for simultaneously detecting antibodies against zoonoses and production diseases in pigs.
In the European Union serological and/or bacteriological monitoring results on zoonoses are to be taken into account for the risk assessment of slaughter pig herds in the framework of the risk-based meat inspection. Furthermore, the European food safety strategy pursues the additional goal to increase herd health and animal welfare. To meet the two goals identifying and controlling the mainly latent zoonoses as well as production diseases, the challenge was to develop and validate a cost-efficient diagnostic tool for simultaneously testing meat juice samples or blood serum samples for antibodies against various pathogens. The following antigens were chosen for detecting the corresponding antibodies by means of a protein microarray: a) zoonotic pathogens (Salmonella spp., Toxoplasma gondii, Trichinella spiralis, Yersinia enterocolitica, hepatitis E virus) and b) production disease causing pathogens (influenza A virus, Mycoplasma hyopneumoniae, PRRSV, Actinobacillus pleuropneumoniae). After validation by reference to the single-ELISA test results seven out of the nine chosen pathogens could be serologically detected by the developed microarray with test accuracy values between 0.71 and 1.